CAG-FNLS-T2A-EGFP-ires-puro
(Plasmid #121169)

• Purpose: CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.
• Depositing Lab: Timo Otonkoski
• Depositing Organization: University of Helsinki
  Located in Finland
• Publication: Otonkoski plasmids 2018 (unpublished)

Backbone

• Vector backbone: PyCAG
• Total vector size (bp): 12644
• Vector type: Mammalian Expression, CRISPR
• Selectable markers: Puromycin ; EGFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FNLS-T2A-EGFP-ires-puro
• Species: Streptococcus pyogenes
• Insert Size (bp): 6042
• Tag / Fusion Protein:
  • T2A-EGFP-ires-puro (C terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: BE3 FNLS fragment cloned from pLenti-FNLS-P2A-GFP-PGK-Puro (Plasmid #110869) from Lukas Dow lab.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 121169-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 121169-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.