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Timo Otonkoski Lab: Otonkoski plasmids 2018
Unpublished

Plasmids from Article

ID Plasmid Purpose  
121169CAG-FNLS-T2A-EGFP-ires-puroCAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.
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121170CAG-ABEmax-P2A-EGFP-ires-puroCAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.
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121171CAG-xFNLS-T2A-EGFP-ires-puroCAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.
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121173FU_tetO_dCas9-VP192-T2A-EGFPLentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.
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121174pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puroDonor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines
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121175pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puroDonor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines
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121176lentiCRISPRv2_PROX1_1+3Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
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121177lentiCRISPRv2_PROX1_1+4Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
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