|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||121173||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Lentiviral, CRISPR ; Inducible by doxycycline
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
/ Fusion Protein
A portion of this plasmid was derived from a plasmid made bydCas9-VP192 cloned from pCXLE-dCas9VP192-T2A-EGFP (Plasmid #69536).
Terms and Licenses
This lentivirus needs to be used together with a doxycycline responsive transcriptional activator expressing lentivirus like FUdeltaGW-rtTA (Plasmid #19780) and lentivirus expressing guide RNAs targeting a promoter for activation (e.g. lentiGuide-Puro
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FU_tetO_dCas9-VP192-T2A-EGFP was a gift from Timo Otonkoski (Addgene plasmid # 121173 ; http://n2t.net/addgene:121173 ; RRID:Addgene_121173)