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(Plasmid #12130)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 12130 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 9761
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    transferrin receptor
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    The portion of the human TfR gene encoding residues 121-760 were fused 3' to a gene segment encoding the hydrophobic leader peptide from the baculovirus protein gp67, a 6X-His tag, and a factor Xa cleavage site in a modified version of the pAcG67A expression vector.
  • GenBank ID
  • Entrez Gene
    TFRC (a.k.a. CD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90)
  • Tags / Fusion Proteins
    • leader peptide from baculovirus protein gp67 (N terminal on insert)
    • 6X-His tag (N terminal on insert)
    • Factor Xa cleavage site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer 5' AAT TTAA AAT gAT AAC CAT CTC gC 3'
  • 3′ sequencing primer 5' gAA gCg ggT CCA AgT TTC CCT g 3'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAcGP67A-TfR was a gift from Pamela Bjorkman (Addgene plasmid # 12130 ; ; RRID:Addgene_12130)
  • For your References section:

    Crystal structure of the hemochromatosis protein HFE and characterization of its interaction with transferrin receptor. Lebron JA, Bennett MJ, Vaughn DE, Chirino AJ, Snow PM, Mintier GA, Feder JN, Bjorkman PJ. Cell. 1998 Apr 3. 93(1):111-23. 10.1016/S0092-8674(00)81151-4 PubMed 9546397