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pLCR0
(Plasmid #12137)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12137 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    na
  • Backbone size (bp) 5010
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer SV40pro-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Arnold J. Levine (Princeton)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid, containing
the previously
described BPV LCR-SV40 hybrid promoter (Spaholz et al., 1985),
was constructed as follows. Plasmid p407-1 (Spaholz et al., 1985) was
digested with EcoRI and HindIII, and the 5010-base pair fragment
containing the LCR-SV40 hybrid promoter was isolated and filled in with Klenow. BamHI linkers were ligated to the ends of the fragment, and the fragment was circularized.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLCR0 was a gift from James Sherley (Addgene plasmid # 12137 ; http://n2t.net/addgene:12137 ; RRID:Addgene_12137)
  • For your References section:

    Guanine nucleotide biosynthesis is regulated by the cellular p53 concentration. Sherley JL. J Biol Chem. 1991 Dec 25. 266(36):24815-28. PubMed 1761576