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pcDNA3-Flag OSM
(Plasmid #12191)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12191 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OSM
  • Alt name
    Ccm2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1362
  • GenBank ID
    AY442689
  • Entrez Gene
    Ccm2 (a.k.a. BC029157)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Flag OSM was a gift from Gary Johnson (Addgene plasmid # 12191 ; http://n2t.net/addgene:12191 ; RRID:Addgene_12191)
  • For your References section:

    Rac-MEKK3-MKK3 scaffolding for p38 MAPK activation during hyperosmotic shock (OSM constructs). Uhlik MT, Abell AN, Johnson NL, Sun W, Cuevas BD, Lobel-Rice KE, Horne EA, Dell'Acqua ML, Johnson GL. Nat Cell Biol. 2003 Dec . 5(12):1104-10. 10.1038/ncb1071 PubMed 14634666