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Addgene

LentiV_Neo_POU2F3_CR
(Plasmid #122175)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122175 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    LentiV_Neo
  • Backbone size w/o insert (bp) 7183
  • Total vector size (bp) 8491
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    POU2F3
  • Alt name
    OCT11
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1308
  • Mutation
    3 silent mutations for gRNA resistance (882g-c, 885t-c, 888g-c)
  • GenBank ID
    NM_014352.4 NM_014352.4
  • Entrez Gene
    POU2F3 (a.k.a. Epoc-1, OCT-11, OCT11, OTF-11, PLA-1, PLA1, Skn-1a)
  • Promoter EFS

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CCGCCACCatggtgaatctggagtccatg
  • 3′ sequencing primer CCGCTGCCgtggaggtaggtggaatgattc
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    DNASU

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LentiV_Neo_POU2F3_CR was a gift from Christopher Vakoc (Addgene plasmid # 122175 ; http://n2t.net/addgene:122175 ; RRID:Addgene_122175)
  • For your References section:

    POU2F3 is a master regulator of a tuft cell-like variant of small cell lung cancer. Huang YH, Klingbeil O, He XY, Wu XS, Arun G, Lu B, Somerville TDD, Milazzo JP, Wilkinson JE, Demerdash OE, Spector DL, Egeblad M, Shi J, Vakoc CR. Genes Dev. 2018 Jul 1;32(13-14):915-928. doi: 10.1101/gad.314815.118. Epub 2018 Jun 26. 10.1101/gad.314815.118 PubMed 29945888