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(Plasmid #122674)


Item Catalog # Description Quantity Price (USD)
Plasmid 122674 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 7809
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    Furin (a.k.a. 9130404I01Rik, Fur, PACE, Pcsk3, SPC1)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GTA ATA CGA CTC ACT ATA GGG
  • 3′ sequencing primer TAG AAG GCA CAG TCG AGG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-mPCSK3 was a gift from Liming Pei (Addgene plasmid # 122674 ; ; RRID:Addgene_122674)
  • For your References section:

    Growth Differentiation Factor 15 Maturation Requires Proteolytic Cleavage by PCSK3, -5, and -6. Li JJ, Liu J, Lupino K, Liu X, Zhang L, Pei L. Mol Cell Biol. 2018 Oct 15;38(21). pii: MCB.00249-18. doi: 10.1128/MCB.00249-18. Print 2018 Nov 1. 10.1128/MCB.00249-18 PubMed 30104250