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Growth Differentiation Factor 15 Maturation Requires Proteolytic Cleavage by PCSK3, -5, and -6.
Li JJ, Liu J, Lupino K, Liu X, Zhang L, Pei L
Mol Cell Biol. 2018 Oct 15;38(21). pii: MCB.00249-18. doi: 10.1128/MCB.00249-18. Print 2018 Nov 1.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
122672pcDNA3.1-mPCSK1Expresses mouse PCSK1 in mammalian cells
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122673pcDNA3.1-mPCSK2Expresses mouse PCSK2 in mammalian cells
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122674pcDNA3.1-mPCSK3Expresses mouse PCSK3 in mammalian cells
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122675pcDNA3.1-mPCSK4Expresses mouse PCSK4 in mammalian cells
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122676pcDNA3.1-mPCSK7Expresses mouse PCSK7 in mammalian cells
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122677pcDNA3.1-mPCSK8Expresses mouse PCSK8 in mammalian cells
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122678pcDNA3.1-mPCSK9Expresses mouse PCSK9 in mammalian cells
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122680pcDNA3.1-mPCSK3 S368AExpresses mutated mouse PCSK3 in mammalian cells (serine at position 368 mutated to alanine via site directed mutagenesis)
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122681pcDNA3.1-V5H6-mPCSK3 D153AExpresses mutated mouse PCSK3 in mammalian cells (aspartic acid at position 153 mutated to alanine via site directed mutagenesis)
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122689pcDNA3.1-mPCSK3 H194LExpresses mutated mouse PCSK3 in mammalian cells (histidine at position 194 mutated to leucine via site directed mutagenesis)
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