Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #122678)


Item Catalog # Description Quantity Price (USD)
Plasmid 122678 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 7512
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Mus musculus proprotein convertase subtilisin/kexin type 9 (Pcsk9)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    Pcsk9 (a.k.a. AI415265, AI747682, FH3, HCHOLA3, Narc1, PC9)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GTA ATA CGA CTC ACT ATA GGG
  • 3′ sequencing primer TAG AAG GCA CAG TCG AGG
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-mPCSK9 was a gift from Liming Pei (Addgene plasmid # 122678 ; ; RRID:Addgene_122678)
  • For your References section:

    Growth Differentiation Factor 15 Maturation Requires Proteolytic Cleavage by PCSK3, -5, and -6. Li JJ, Liu J, Lupino K, Liu X, Zhang L, Pei L. Mol Cell Biol. 2018 Oct 15;38(21). pii: MCB.00249-18. doi: 10.1128/MCB.00249-18. Print 2018 Nov 1. 10.1128/MCB.00249-18 PubMed 30104250