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pAR17_StrepI_hFASh_H8_pET22b
(Plasmid #122846)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 122846 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET22b
  • Backbone manufacturer
    Novagen/Merck Millipore
  • Backbone size w/o insert (bp) 5340
  • Total vector size (bp) 13008
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    human FASN/ human fatty acid synthase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    7668
  • Mutation
    wildtype
  • Entrez Gene
    FASN (a.k.a. FAS, OA-519, SDR27X1)
  • Promoter T7 promoter
  • Tags / Fusion Proteins
    • Twin-Strep tag (N terminal on insert)
    • His8 tag (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 prom
  • 3′ sequencing primer T7 term
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAR17_StrepI_hFASh_H8_pET22b was a gift from Martin Grininger (Addgene plasmid # 122846 ; http://n2t.net/addgene:122846 ; RRID:Addgene_122846)
  • For your References section:

    Probing the modularity of megasynthases by rational engineering of a fatty acid synthase Type I. Rittner A, Paithankar KS, Drexler DJ, Himmler A, Grininger M. Protein Sci. 2019 Feb;28(2):414-428. doi: 10.1002/pro.3550. Epub 2018 Dec 20. 10.1002/pro.3550 PubMed 30394635