Probing the modularity of megasynthases by rational engineering of a fatty acid synthase Type I.
Rittner A, Paithankar KS, Drexler DJ, Himmler A, Grininger M
Protein Sci. 2019 Feb;28(2):414-428. doi: 10.1002/pro.3550. Epub 2018 Dec 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 122846 | pAR17_StrepI_hFASh_H8_pET22b | Expresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector. |
| 122847 | pAR18_StrepI_mFASm_H8_pET22b | Expresses murine type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector. |
| 122849 | pAR69_STRI_m(KS_MAT)_H8_pET22b | Expresses the condensing part of murine type I fatty acid synthase (FASN) consisting of the KS and MAT domain in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector. |
| 122850 | pAR127_StrepI_m(KS_MAT)_mouseL_ACP_H8_pET22b | Expresses a designed construct of the condensing part fused to the ACP domain of murine type I fatty acid synthase (FASN) in Escherichia coli |
| 122851 | pAR357_SFP_pCDF-1b | Co-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector. |