PurposeExpresses murine type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||122847||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerNovagen/Merck Millipore
- Backbone size w/o insert (bp) 5340
- Total vector size (bp) 12987
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberLow Copy
Gene/Insert namemouse FASN/ mouse fatty acid synthase
SpeciesM. musculus (mouse)
Insert Size (bp)7647
Entrez GeneFasn (a.k.a. A630082H08Rik, FAS)
- Promoter T7 promoter
/ Fusion Proteins
- Twin-Strep tag (N terminal on insert)
- His8 tag (C terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7 prom
- 3′ sequencing primer T7 term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAR18_StrepI_mFASm_H8_pET22b was a gift from Martin Grininger (Addgene plasmid # 122847 ; http://n2t.net/addgene:122847 ; RRID:Addgene_122847)
For your References section:Probing the modularity of megasynthases by rational engineering of a fatty acid synthase Type I. Rittner A, Paithankar KS, Drexler DJ, Himmler A, Grininger M. Protein Sci. 2019 Feb;28(2):414-428. doi: 10.1002/pro.3550. Epub 2018 Dec 20. 10.1002/pro.3550 PubMed 30394635