pBluescript SK +/- mTRF1
(Plasmid #12303)

• Depositing Lab: Titia de Lange
• Publication: Broccoli et al Hum Mol Genet. 1997 Jan . 6(1):69-76.

Backbone

• Vector backbone: pBluescript SK +/-
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mTRF1
• Alt name: mouse TTAGGG repeat binding factor 1
• Alt name: 840
• Species: M. musculus (mouse)
• Insert Size (bp): 1700
• GenBank ID: u65586
• Entrez Gene: Terf1 (a.k.a. Pin2, Trbf1, Trf1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry