PurposeBaculovirus expression vector for HAP40 protein (aa 1-371) in insect cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||124060||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerArrowsmith lab (Addgene plasmid# 62304)
- Backbone size w/o insert (bp) 6767
Vector typeBaculovirus expression
Growth in Bacteria
SpeciesH. sapiens (human)
Insert Size (bp)1113
Entrez GeneF8A1 (a.k.a. DXS522E, F8A, HAP40)
- Promoter polyhedrin
/ Fusion Proteins
- 6x His (N terminal on backbone)
- TEV cleavage site (N terminal on backbone)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer AGTATTTTACTGTTTTCG
- 3′ sequencing primer TAACCATTATAAGCTGC (Common Sequencing Primers)
Terms and Licenses
Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HAP40 1-371 was a gift from Cheryl Arrowsmith (Addgene plasmid # 124060 ; http://n2t.net/addgene:124060 ; RRID:Addgene_124060)
For your References section:Design and characterization of mutant and wildtype huntingtin proteins produced from a toolkit of scalable eukaryotic expression systems. Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith CH. J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 10.1074/jbc.RA118.007204. Epub 2019 Mar 6. 10.1074/jbc.RA118.007204 PubMed 30842263