Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Design and characterization of mutant and wildtype huntingtin proteins produced from a toolkit of scalable eukaryotic expression systems.
Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith CH
J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
111723pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q23Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111724pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q73Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111725pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q79Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111726pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q23Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111727pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q54Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111728pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q73Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111729pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q79Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111730pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q109Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111731pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q145Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111732pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q15Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111733pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q19Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111734pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q51Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111735pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q66Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111736pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q85Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111737pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q78Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111738pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q139Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111741pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q19Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111742pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q24Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111743pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q25Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111744pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q30Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111745pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q36Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111746pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q42Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111747pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q48Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111748pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q52Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111749pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q60Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111750pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q66Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
Loading...
111751pBacMam2-DiEx-LIC-N entry vectorIntermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with N-term FLAG tag. Baculovirus transfer vector for insect and mammalian cells
Loading...
111752pBacMam2-DiEx-LIC-C entry vectorIntermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with C-term FLAG tag. Baculovirus transfer vector for insect and mammalian cells
Loading...
124060HAP40 1-371Baculovirus expression vector for HAP40 protein (aa 1-371) in insect cells
Loading...