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pBacMam2-DiEx-LIC-C entry vector
(Plasmid #111752)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 111752 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBacMam2-DiEx-LIC
  • Backbone size (bp) 15911
  • Vector type
    Mammalian Expression ; Baculovirus Expression
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains most of the HTT gene: amino acids (AA) 1-6, a cloning site, and AA 131-3144. It lacks the polyQ region so that other polyQ lengths can be introduced. Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBacMam2-DiEx-LIC-C entry vector was a gift from Cheryl Arrowsmith (Addgene plasmid # 111752 ; http://n2t.net/addgene:111752 ; RRID:Addgene_111752)
  • For your References section:

    Design and characterization of mutant and wildtype huntingtin proteins produced from a toolkit of scalable eukaryotic expression systems. Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith CH. J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 10.1074/jbc.RA118.007204. Epub 2019 Mar 6. 10.1074/jbc.RA118.007204 PubMed 30842263