pLenti6.3-KLRC1-V5
(Plasmid
#124093)
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PurposeKLRC1-V5 tagged expression vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124093 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepLenti6.3
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Backbone manufacturerThermofisher
- Backbone size w/o insert (bp) 7692
- Total vector size (bp) 8394
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Vector typeLentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameKLRC1
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Alt namekiller cell lectin like receptor C1
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SpeciesH. sapiens (human)
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Insert Size (bp)702
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Entrez GeneKLRC1 (a.k.a. CD159A, NKG2, NKG2A)
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti6.3-KLRC1-V5 was a gift from J. Chad Brenner (Addgene plasmid # 124093 ; http://n2t.net/addgene:124093 ; RRID:Addgene_124093) -
For your References section:
Genome-wide open reading frame profiling identifies fibroblast growth factor signaling as a driver of PD-L1 expression in head and neck squamous cell carcinoma. Mann JE, Smith JD, Kulkarni A, Foltin SK, Scheftz EB, Murray IR, Gensterblum-Miller E, Brummel CV, Bhangale A, Hoesli RC, Brenner JC. Oral Oncol. 2023 Nov;146:106562. doi: 10.1016/j.oraloncology.2023.106562. Epub 2023 Sep 2. 10.1016/j.oraloncology.2023.106562 PubMed 37666053