pAAV-CMV-BDNF-Myc
(Plasmid
#124279)
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PurposeExpress Myc-tagged brain-derived neurotrophic factor (BDNF)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 124279 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAAV-MCS
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Backbone manufacturerAgilent
- Backbone size w/o insert (bp) 4600
- Total vector size (bp) 7338
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Modifications to backboneThe sequence between two NotI sites is replaced with a sequence containing the CMV promoter, BDNF coding region, Myc tag and BDNF 3' untranslated region.
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBDNF
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SpeciesM. musculus (mouse)
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Insert Size (bp)3600
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GenBank IDAY057907
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Entrez GeneBdnf
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Tag
/ Fusion Protein
- Myc (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-CMV-BDNF-Myc was a gift from Baoji Xu (Addgene plasmid # 124279 ; http://n2t.net/addgene:124279 ; RRID:Addgene_124279) -
For your References section:
Activation of Anxiogenic Circuits Instigates Resistance to Diet-Induced Obesity via Increased Energy Expenditure. Xie X, Yang H, An JJ, Houtz J, Tan JW, Xu H, Liao GY, Xu ZX, Xu B. Cell Metab. 2019 Jan 11. pii: S1550-4131(18)30755-1. doi: 10.1016/j.cmet.2018.12.018. 10.1016/j.cmet.2018.12.018 PubMed 30661931
Map uploaded by the depositor.
