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(Plasmid #124296)


Item Catalog # Description Quantity Price (USD)
Plasmid 124296 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5237
  • Total vector size (bp) 6500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
  • Species
  • Insert Size (bp)
  • Tag / Fusion Protein
    • His6 and T7 tags (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

T7 promoter so expression requires DE3 strain. Pilot experiments using E. coli BL21(DE3) indicate 18C expression overnight gives higher yields than 37C (for 4 hours). The fusion protein was soluble and purified by Ni-NTA chromatography using 50 mM Tris pH 8.0, 300 mM NaCl, 10/300 mM imidazole. Size exclusion chromatography indicated that the fusion protein was tetrameric in Tris-buffered saline; no aggregates were present.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29b-H6_Streptavidin_sfGFP was a gift from Mark Arbing (Addgene plasmid # 124296 ; ; RRID:Addgene_124296)