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pUHD-HA-AML1-ETO
(Plasmid #12430)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12430 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AML1-ETO
  • Alt name
    AML1
  • Alt name
    ETO
  • Species
    H. sapiens (human)
  • Mutation
    BstXI-SacII fragment in pUHD-AML1-ETO replaced with HA tagged fragment
  • Entrez Gene
    RUNX1 (a.k.a. AML1, AML1-EVI-1, AMLCR1, CBF2alpha, CBFA2, EVI-1, PEBP2aB, PEBP2alpha)
  • Entrez Gene
    RUNX1T1 (a.k.a. AML1-MTG8, AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer LNCX
  • 3′ sequencing primer EBV rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUHD-HA-AML1-ETO was a gift from Dong-Er Zhang (Addgene plasmid # 12430 ; http://n2t.net/addgene:12430 ; RRID:Addgene_12430)
  • For your References section:

    Analysis of the role of AML1-ETO in leukemogenesis, using an inducible transgenic mouse model. Rhoades KL, Hetherington CJ, Harakawa N, Yergeau DA, Zhou L, Liu LQ, Little MT, Tenen DG, Zhang DE. Blood. 2000 Sep 15. 96(6):2108-15. PubMed 10979955