pGEX-C/EBPbZIP (pGEX-bZIPa)
(Plasmid
#12435)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12435 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEX-2TK
- Backbone size w/o insert (bp) 5000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name68 a.a. fragment of C/EBP alpha
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Alt nameCEBPA
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SpeciesH. sapiens (human)
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Insert Size (bp)204
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Entrez GeneCEBPA (a.k.a. C/EBP-alpha, CEBP)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pGEX 5'
- 3′ sequencing primer pGEX 3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
68 a.a. of human C/EBPa in frame with GST in BamHI and EcoRI sites of pGEX-2TK.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-C/EBPbZIP (pGEX-bZIPa) was a gift from Dong-Er Zhang (Addgene plasmid # 12435 ; http://n2t.net/addgene:12435 ; RRID:Addgene_12435) -
For your References section:
CCAAT enhancer-binding protein (C/EBP) and AML1 (CBF alpha2) synergistically activate the macrophage colony-stimulating factor receptor promoter. Zhang DE, Hetherington CJ, Meyers S, Rhoades KL, Larson CJ, Chen HM, Hiebert SW, Tenen DG. Mol Cell Biol. 1996 Mar . 16(3):1231-40. PubMed 8622667