Gal4-TET1-878-911-WT(pL84)
(Plasmid
#124400)
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PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of WT TET1 SID.
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Depositing Lab
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Depositing OrganizationUniversity of Leicester
Located in the United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 124400 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 124400-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 124400-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepLeicester 84
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Backbone manufacturerUniversity of Leicester
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGal4-TET1-878-911 wildtype
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SpeciesH. sapiens (human)
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Insert Size (bp)228
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Entrez GeneTET1 (a.k.a. CXXC6, LCX, bA119F7.1)
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Tag
/ Fusion Protein
- gal4 dna binding domain (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 124400-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 124400-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Gal4-TET1-878-911-WT(pL84) was a gift from Shaun Cowley (Addgene plasmid # 124400 ; http://n2t.net/addgene:124400 ; RRID:Addgene_124400) -
For your References section:
Sin3A recruits Tet1 to the PAH1 domain via a highly conserved Sin3-Interaction Domain. Chandru A, Bate N, Vuister GW, Cowley SM. Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.1038/s41598-018-32942-w. 10.1038/s41598-018-32942-w PubMed 30279502
Map uploaded by the depositor.
