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Addgene
Sin3A recruits Tet1 to the PAH1 domain via a highly conserved Sin3-Interaction Domain.
Chandru A, Bate N, Vuister GW, Cowley SM
Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.1038/s41598-018-32942-w. PubMed Article

Plasmids from Article

ID Plasmid Purpose
124386His-Sin3a-115-212(pL01)E.Coli produced SIN3A PAH1 purified by FPLC nickel column
124387GB1-His-SAP25-126-186(pL91)E.Coli produced SAP25 SID purified by FPLC nickel column
124388GB1-His-TET1-848-952(pL91)E.Coli produced TET1-848-952 purified by FPLC nickel column
124390GB1-His-TET1-878-911(pL91)E.Coli produced TET1 SID (TET1-878-911) purified by FPLC nickel column
124391GB1-His-TET1-878-911-I894A(pL91)E.Coli produced TET1-SID mutants (I894A) purified by FPLC nickel column
124392GB1-His-TET1-878-911-L897A(pL91)E.Coli produced TET1-SID mutants (L897A) purified by FPLC nickel column
124393GB1-His-TET1-878-911-T898A(pL91)E.Coli produced TET1-SID mutants (T898A) purified by FPLC nickel column
124394GB1-His-TET1-878-911-T898E(pL91)E.Coli produced TET1-SID mutants (T898E) purified by FPLC nickel column
124395GB1-His-TET1-878-911-L900A(pL91)E.Coli produced TET1-SID mutants (L900A) purified by FPLC nickel column
124396FLAG-FullLength-TET1-WT(21)Full-length TET1 used to PiggyBAC rescue the function of TET1/2 DKO cells
124397FLAG-FullLength-TET1-LLAA(20)Full-length mutant TET1 (L897A/L900A) used to PiggyBAC rescue the function of TET1/2 DKO cells
124398FLAG-FullLength-TET1-CXXC(24)Full-length TET1 missing zinc finger domain (585-624) used to PiggyBAC rescue the function of TET1/2 DKO cells
124399FLAG-FullLength-TET1-CXXC/LLAAFull-length mutant TET1 (L897A/L900A), missing zinc finger domain (585-624), used to PiggyBAC rescue the function of TET1/2 DKO cells
124400Gal4-TET1-878-911-WT(pL84)Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of WT TET1 SID.
124401Gal4-TET1-878-911-L897A(pL84)Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L897A mutation.
124402Gal4-TET1-878-911-L900A(pL84)Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L900A mutation.
124403Gal4-TET1-878-911-T898A(pL84)Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with T898A mutation.
124404Gal4-TET1-878-911-I894A(pL84)Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with I894A mutation.
124406FLAG-TET1-746-951(pL72)TET1-746-951, containing SID, used for Co-IP.

Antibodies from Article