Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12455||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerBruce Mayer
- Backbone size w/o insert (bp) 6100
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
MutationpEBG vector is from Bruce Mayer, may be different from other pEBG vectors in public domain.
Entrez GeneUSP18 (a.k.a. ISG43, PTORCH2, UBP43)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pGEX5' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEBG-hUBP43 was a gift from Dong-Er Zhang (Addgene plasmid # 12455 ; http://n2t.net/addgene:12455 ; RRID:Addgene_12455)
For your References section:UBP43 is a novel regulator of interferon signaling independent of its ISG15 isopeptidase activity. Malakhova OA, Kim KI, Luo JK, Zou W, Kumar KG, Fuchs SY, Shuai K, Zhang DE. EMBO J. 2006 Jun 7. 25(11):2358-67. 10.1038/sj.emboj.7601149 PubMed 16710296