PurposeExpressed barcode for sample multiplexing in single-cell analysis
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||124591||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 8426
- Total vector size (bp) 8432
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Insert Size (bp)8
- Promoter hCMV
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer SV40 PolyA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The pSMAL backbone was a kind gift from John Dick: van Galen, P. et al. The unfolded protein response governs integrity of the haematopoietic stem-cell pool during stress. Nature 510, 268–72 (2014).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CellTagA was a gift from Samantha Morris (Addgene plasmid # 124591 ; http://n2t.net/addgene:124591 ; RRID:Addgene_124591)