B4GALT1-ABA
(Plasmid #124639)

• Purpose: Abscisic acid-inducible B4GALT1 expression in mammalian cells
• Depositing Lab: Ron Weiss
• Publication: Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038/s41589-019-0288-4. doi: 10.1038/s41589-019-0288-4.

Backbone

• Vector backbone: pJAZZ
• Backbone manufacturer: Lucigen
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Beta-(1,4)-Galactosyltransferase 1
• Alt name: b4GALT1
• Species: Cricetulus griseus
• Insert Size (bp): 1182
• Promoter: PhlF-activatable promoter (6x PhlF Operators + miniCMV promoter)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GGCACAGTCAATGAAGTTTGAAAACCAATAAGAGCTCGGTAAACC
• 3′ sequencing primer: GGATCTTAAAAACATTATACAATACTACAAGCATAAAAACGCACC

Gene/Insert 2

• Gene/Insert name: NLS-VP16-PYL1
• Insert Size (bp): 831
• Promoter: hEF1a

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GGTGCGTTTTTATGCTTGTAGTATTGTATAATGTTTTTAAGATCC
• 3′ sequencing primer: GGCGTATAAAACATCTGGATAAGACGAGAGATTGGGTATTAGACC

Gene/Insert 3

• Gene/Insert name: PhlF(activator)-NES-Gal4-ABI
• Insert Size (bp): 1572
• Promoter: hEF1a

Cloning Information for Gene/Insert 3

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GGTCTAATACCCAATCTCTCGTCTTATCCAGATGTTTTATACGCC
• 3′ sequencing primer: GGGACAAACTCGCCTGCCTTTTACACTCACATAAGGGAATTCACC

Gene/Insert 4

• Gene/Insert name: TagFP635
• Alt name: mKate
• Insert Size (bp): 713
• Promoter: hEF1a

Cloning Information for Gene/Insert 4

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GGTGAATTCCCTTATGTGAGTGTAAAAGGCAGGCGAGTTTGTCCC
• 3′ sequencing primer: GGATTCTTTGAGAGTGCTTTCCAATTACTGCTTTTGCAAGCAACC

Resource Information

• Supplemental Documents:
  • B4GALT1-ABA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry