B4GALT1-ABA
(Plasmid
#124639)
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PurposeAbscisic acid-inducible B4GALT1 expression in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 124639 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepJAZZ
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Backbone manufacturerLucigen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameBeta-(1,4)-Galactosyltransferase 1
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Alt nameb4GALT1
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SpeciesCricetulus griseus
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Insert Size (bp)1182
- Promoter PhlF-activatable promoter (6x PhlF Operators + miniCMV promoter)
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer GGCACAGTCAATGAAGTTTGAAAACCAATAAGAGCTCGGTAAACC
- 3′ sequencing primer GGATCTTAAAAACATTATACAATACTACAAGCATAAAAACGCACC (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameNLS-VP16-PYL1
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Insert Size (bp)831
- Promoter hEF1a
Cloning Information for Gene/Insert 2
- Cloning method Gibson Cloning
- 5′ sequencing primer GGTGCGTTTTTATGCTTGTAGTATTGTATAATGTTTTTAAGATCC
- 3′ sequencing primer GGCGTATAAAACATCTGGATAAGACGAGAGATTGGGTATTAGACC (Common Sequencing Primers)
Gene/Insert 3
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Gene/Insert namePhlF(activator)-NES-Gal4-ABI
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Insert Size (bp)1572
- Promoter hEF1a
Cloning Information for Gene/Insert 3
- Cloning method Gibson Cloning
- 5′ sequencing primer GGTCTAATACCCAATCTCTCGTCTTATCCAGATGTTTTATACGCC
- 3′ sequencing primer GGGACAAACTCGCCTGCCTTTTACACTCACATAAGGGAATTCACC (Common Sequencing Primers)
Gene/Insert 4
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Gene/Insert nameTagFP635
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Alt namemKate
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Insert Size (bp)713
- Promoter hEF1a
Cloning Information for Gene/Insert 4
- Cloning method Gibson Cloning
- 5′ sequencing primer GGTGAATTCCCTTATGTGAGTGTAAAAGGCAGGCGAGTTTGTCCC
- 3′ sequencing primer GGATTCTTTGAGAGTGCTTTCCAATTACTGCTTTTGCAAGCAACC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
B4GALT1-ABA was a gift from Ron Weiss (Addgene plasmid # 124639) -
For your References section:
Small-molecule control of antibody N-glycosylation in engineered mammalian cells. Chang MM, Gaidukov L, Jung G, Tseng WA, Scarcelli JJ, Cornell R, Marshall JK, Lyles JL, Sakorafas P, Chu AA, Cote K, Tzvetkova B, Dolatshahi S, Sumit M, Mulukutla BC, Lauffenburger DA, Figueroa B Jr, Summers NM, Lu TK, Weiss R. Nat Chem Biol. 2019 May 20. pii: 10.1038/s41589-019-0288-4. doi: 10.1038/s41589-019-0288-4. 10.1038/s41589-019-0288-4 PubMed 31110306