pD21-hgAPOAI
(Plasmid
#124962)
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PurposeExpress ApoAI in the liver
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 124962 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepD21
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Backbone manufacturerBCM
- Backbone size w/o insert (bp) 24000
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Vector typeAdenoviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman APOAI gene
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SpeciesH. sapiens (human)
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Entrez GeneLPA (a.k.a. AK38, APOA, LP)
- Promoter human APOAI
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note plasmid was difficult to sequence due to its size. Addgene NGS results contain several ambiguous bases. We advise running additional diagnostic tests before use.
The backbone was developed at BCM. The relevant reference is Proc. Natl. Acad Sci. USA 98: 13282-13287.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pD21-hgAPOAI was a gift from Kazuhiro Oka (Addgene plasmid # 124962 ; http://n2t.net/addgene:124962 ; RRID:Addgene_124962) -
For your References section:
Long-term stable expression of human apolipoprotein A-I mediated by helper-dependent adenovirus gene transfer inhibits atherosclerosis progression and remodels atherosclerotic plaques in a mouse model of familial hypercholesterolemia. Belalcazar LM, Merched A, Carr B, Oka K, Chen KH, Pastore L, Beaudet A, Chan L. Circulation. 2003 Jun 3;107(21):2726-32. doi: 10.1161/01.CIR.0000066913.69844.B2. Epub 2003 May 12. 10.1161/01.CIR.0000066913.69844.B2 PubMed 12742997
