6379 H10-K218T
(Plasmid #1250)

• Depositing Lab: Susan Lindquist
• Publication: Glover et al Cell 1998 Jul 10;94(1):73-82.

Backbone

• Vector backbone: pJC45S
• Modifications to backbone: *See Comments section below
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Unknown
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Hsp104 (K218T)
• Alt name: Hsp104
• Species: S. cerevisiae (budding yeast)
• Mutation: K218T
• Entrez Gene: HSP104 (a.k.a. YLL026W)
• Tag / Fusion Protein:
  • his tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: John Glover

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene comment: Please note that the plasmid backbone does not match the pJC45 backbone. The depositor described this plasmid as having a "pJC45S" backbone. Full plasmid sequencing shows features similar to other yeast expression vectors. The lab is closed so were were unable to ask if this difference was intended.