RE642
(Bacterial strain
#125019)
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PurposeRecombineering rate estimation. Strain name: GC/K@SS4
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Depositing Labs
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Sequence Information
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Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Bacterial Strain | 125019 | Bacteria in agar stab | 1 | $89 | |
Backbone
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Vector backbonenone
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)This is a strain.
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Copy numberUnknown
Depositor Comments
Genotype: pTet2-gam-bet-exo-dam/tetR/ampR::bioA/B ilvG+ lacIQ1 Pcp8-araE ΔaraBAD pConst-araC dnaG.Q576A ΔrecJ ΔxonA
Integrated Cassette: sfGFP-cmR+mKate2::SS4
Host Strain: BioDesignER
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RE642 was a gift from (Addgene plasmid # 125019) -
For your References section:
A versatile platform strain for high-fidelity multiplex genome editing. Egbert RG, Rishi HS, Adler BA, McCormick DM, Toro E, Gill RT, Arkin AP. Nucleic Acids Res. 2019 Feb 21. pii: 5351610. doi: 10.1093/nar/gkz085. 10.1093/nar/gkz085 PubMed 30788501