|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12512||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size (bp) 7218
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site See map (unknown if destroyed)
- 3′ cloning site See map (unknown if destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
pCMV-TO4 has four copies of the tet operator site tetO2 and multiple cloning sites cloned downstream to a CMV promoter. An MluI fragment containing the TRSID expression cassette (CMV-TRSID-IRES-Neo-SV40 PA) was subcloned into the pCMV-TO4 vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTHE was a gift from Tong-Chuan He (Addgene plasmid # 12512 ; http://n2t.net/addgene:12512 ; RRID:Addgene_12512)
For your References section:Tetracycline-regulated gene expression mediated by a novel chimeric repressor that recruits histone deacetylases in mammalian cells. Jiang W, Zhou L, Breyer B, Feng T, Cheng H, Haydon R, Ishikawa A, He TC. J Biol Chem. 2001 Nov 30. 276(48):45168-74. 10.1074/jbc.M106924200 PubMed 11581265