pGL3_4xUAS_CP-candidate_luc+
(Plasmid
#125154)
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PurposeSTAP-seq luciferase validation vector, which harbors an 4x UAS array upstream of the CP position (candidates are inserted at CP position) for the recruitement of GAL4-DBD tagged proteins
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Depositing Lab
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Depositing OrganizationResearch Institute of Molecular Pathology (IMP)
Located in Austria -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 125154 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 125154-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 125154-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneUnknown
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4779
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name4 x upstream activating sequence (UAS)
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)177
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 125154-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 125154-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL3_4xUAS_CP-candidate_luc+ was a gift from Alexander Stark (Addgene plasmid # 125154 ; http://n2t.net/addgene:125154 ; RRID:Addgene_125154) -
For your References section:
Transcriptional cofactors display specificity for distinct types of core promoters. Haberle V, Arnold CD, Pagani M, Rath M, Schernhuber K, Stark A. Nature. 2019 Jun;570(7759):122-126. doi: 10.1038/s41586-019-1210-7. Epub 2019 May 15. 10.1038/s41586-019-1210-7 PubMed 31092928
