Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12518||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9220
Vector typeMammalian Expression, Adenoviral
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Entrez GeneWnt3a (a.k.a. Wnt-, Wnt-3a, vt)
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAd-Wnt3a was a gift from Tong-Chuan He (Addgene plasmid # 12518 ; http://n2t.net/addgene:12518 ; RRID:Addgene_12518)
For your References section:Connective tissue growth factor (CTGF) is regulated by Wnt and bone morphogenetic proteins signaling in osteoblast differentiation of mesenchymal stem cells. Luo Q, Kang Q, Si W, Jiang W, Park JK, Peng Y, Li X, Luu HH, Luo J, Montag AG, Haydon RC, He TC. J Biol Chem. 2004 Dec 31. 279(53):55958-68. 10.1074/jbc.M407810200 PubMed 15496414