FD1
(Bacterial strain #125258)

• Purpose: Derivative of MG1655 used for the screening of clones able to survive the bad seed toxicity of dCas9, while still efficiently silencing the expression of mcherry
• Depositing Lab: David Bikard
• Publication: Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)30497-3. doi: 10.1016/j.ymeth.2019.07.024.

Backbone

• Vector backbone: none

Growth in Bacteria

• Bacterial Resistance(s): None
• Growth Temperature: 37°C
• Growth Strain(s): This is a strain.
• Growth instructions: Grows on LB medium
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: single chromosomal copy of yhhX target sequence upstream from mcherry reporter gene under control of a constitutive promoter

Resource Information

• Supplemental Documents:
  • Strain Verification Data

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Genotype:

The expression of mcherry can be visualized or measured with a spectrophotometer.

The yhhX target sequence followed by a mcherry reporter gene carried by an integrative vector based on pOSIP-KL was integrated at the lambda attB site in the chromosome of E. coli MG1655 and the backbone was flipped out using the pE-FLP (AmpR, #45978, Addgene) plasmid.

This strain can be used to optimize dCas9 expression levels