pRS1422
(Plasmid #12548)

• Depositing Lab: Robert Sobol
• Publication: Trivedi et al Cancer Res. 2005 Jul 15. 65(14):6394-400.

Backbone

• Vector backbone: pIRESNeo
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5255
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human Methylpurine DNA glycosylase
• Alt name: MPG
• Alt name: AAG
• Species: H. sapiens (human)
• Insert Size (bp): 900
• GenBank ID: NM_002434
• Entrez Gene: MPG (a.k.a. AAG, ADPG, APNG, CRA36.1, MDG, Mid1, PIG11, PIG16, anpg)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 3′ cloning site: EcoRV (destroyed during cloning)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Mpg cDNA originally obtained from Leona Samson, MIT.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry