pCAFNF-PSDd1.2-GFP
(Plasmid
#125581)
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PurposeFlp-dependent expression of PSD d1.2-GFP (a deletion mutant of PSD-95 fused to GFP) in mammalian cells.
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Depositing Lab
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Depositing OrganizationKyushu University
Located in Japan -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 125581 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 125581-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 125581-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCAFNF
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePSDd1.2-GFP
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Alt namePSD95 deletion mutant
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Alt nameDlg4
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SpeciesM. musculus (mouse)
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Entrez GeneDlg4 (a.k.a. Dlgh4, PSD-95, PSD95, SAP90, SAP90A)
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made by13385pCALNL vector is derived from Addgene #13770. EGFP is from Clontech.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PSDd1.2-GFP was described in PMID: 10402201.
Arnold, D. B. & Clapham, D. E.Molecular determinants for subcellular localizationof PSD-95 with an interacting K1 channel. Neuron 23, 149–157 (1999).
Please visit https://www.biorxiv.org/content/10.1101/625616v2 for bioRxiv preprint.
DNA (Catalog # 125581-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 125581-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAFNF-PSDd1.2-GFP was a gift from Takeshi Imai (Addgene plasmid # 125581 ; http://n2t.net/addgene:125581 ; RRID:Addgene_125581) -
For your References section:
Activity-dependent local protection and lateral inhibition control synaptic competition in developing mitral cells in mice. Fujimoto S, Leiwe MN, Aihara S, Sakaguchi R, Muroyama Y, Kobayakawa R, Kobayakawa K, Saito T, Imai T. Dev Cell. 2023 Jul 24;58(14):1221-1236.e7. doi: 10.1016/j.devcel.2023.05.004. Epub 2023 Jun 7. 10.1016/j.devcel.2023.05.004 PubMed 37290446
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Map uploaded by the depositor.
