pJin-290
(Plasmid
#125718)
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PurposeABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19 with CGG RNAP-C
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Depositing Lab
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Depositing OrganizationUniversity of Chicago
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 125718 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 125718-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 125718-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepBR322
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePCGG-gRNA GFP-off expression, FKBP-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-FRB
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gRNA/shRNA sequenceunknown
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SpeciesH. sapiens (human)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byPYL/ABI genes were cloned from Prof. Liang's vector. ( ref. Liang, F. S., Ho, W. Q., and Crabtree, G. R. (2011) Engineering the ABA plant stress pathway for regulation of induced proximity. Sci. Signaling 4, rs2.)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 125718-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 125718-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJin-290 was a gift from Bryan Dickinson (Addgene plasmid # 125718 ; http://n2t.net/addgene:125718 ; RRID:Addgene_125718) -
For your References section:
Multidimensional Control of Cas9 by Evolved RNA Polymerase-Based Biosensors. Pu J, Kentala K, Dickinson BC. ACS Chem Biol. 2018 Feb 16;13(2):431-437. doi: 10.1021/acschembio.7b00532. Epub 2017 Aug 22. 10.1021/acschembio.7b00532 PubMed 28809467
