GEV 2
(Plasmid #12616)

• Depositing Lab: John Louis
• Publication: Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

Backbone

• Vector backbone: pET21a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5443
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GB1 domain of protein G as N terminal tag, with IgT antigen as insert
• Alt name: GB1 domain of protein G
• Alt name: IgT (aa 131-259)
• Alt name: SV40gp6
• Species: SV40
• Insert Size (bp): 384
• Entrez Gene: SV40gp6 (a.k.a. SV40gp6)
• Tags / Fusion Proteins:
  • G protein (N terminal on backbone)
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI, Sac I, Sal I, Eag I, Not I, Xho I (not destroyed)
• 5′ sequencing primer: T7 terminal

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The GEV2 vector contains a linker (LVPRGS) with a
thrombin cleavage site between the GB1 domain and the N-terminus of the protein of interest. Genes are inserted between a BamHI site at the 5'
end and several possible restriction sites at the 3' end. Cloning into the XhoI site allows incorporation of a poly His-tag.

Also see: "A rapid method to attain isotope labeled small soluble peptides for NMR studies", Journal of Biomolecular NMR, Vol 26, No 3, July 2003.