Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12630||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepGEX 2T
- Backbone size w/o insert (bp) 5000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1275
Entrez GeneSMAD3 (a.k.a. HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pGEX 5'
- 3′ sequencing primer pGEX 3' (Common Sequencing Primers)
See author's map for details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEX Smad3 was a gift from Rik Derynck (Addgene plasmid # 12630 ; http://n2t.net/addgene:12630 ; RRID:Addgene_12630)
For your References section:Receptor-associated Mad homologues synergize as effectors of the TGF-beta response. Zhang Y, Feng X, We R, Derynck R. Nature. 1996 Sep 12. 383(6596):168-72. 10.1038/383168a0 PubMed 8774881
Map uploaded by the depositor.