pGEX Smad3 NL
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12632||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameSmad3 NL
SpeciesH. sapiens (human)
Insert Size (bp)633
MutationN-L segment of Smad3 (aa 1-211)
Entrez GeneSMAD3 (a.k.a. HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer pGEX 5'
- 3′ sequencing primer pGEX 3' (Common Sequencing Primers)
Terms and Licenses
See author's map for details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEX Smad3 NL was a gift from Rik Derynck (Addgene plasmid # 12632 ; http://n2t.net/addgene:12632 ; RRID:Addgene_12632)
For your References section:Smad3 and Smad4 cooperate with c-Jun/c-Fos to mediate TGF-beta-induced transcription. Zhang Y, Feng XH, Derynck R. Nature. 1998 Aug 27. 394(6696):909-13. 10.1038/29814 PubMed 9732876
Map uploaded by the depositor.