pCDNA3 V5 mDispatched WT 2x HA
(Plasmid
#126410)
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PurposeExpression of V5 Dispatched WT HA in mammalian cell lines.
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Depositing Lab
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Depositing OrganizationSt. Jude Children's Research Hospital
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 126410 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 126410-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 126410-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namem-Dispatched1
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SpeciesM. musculus (mouse)
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MutationV5 iserted between A106 and A107
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Entrez GeneDisp1 (a.k.a. 1190008H24Rik, DispA)
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 126410-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 126410-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3 V5 mDispatched WT 2x HA was a gift from Stacey Ogden (Addgene plasmid # 126410 ; http://n2t.net/addgene:126410 ; RRID:Addgene_126410) -
For your References section:
Cleavage activates dispatched for Sonic Hedgehog ligand release. Stewart DP, Marada S, Bodeen WJ, Truong A, Sakurada SM, Pandit T, Pruett-Miller SM, Ogden SK. Elife. 2018 Jan 23;7. pii: 31678. doi: 10.7554/eLife.31678. 31678 [pii] PubMed 29359685
