SHIP2 WT pBABE-His-Xpress
(Plasmid #126584)

• Purpose: Retroviral infection of MEF cells (puromycin selection)
• Depositing Lab: Christophe Erneux
• Publication: Zhang et al Cell Signal. 2007 Oct;19(10):2194-200. doi: 10.1016/j.cellsig.2007.06.022. Epub 2007 Jun 30.

Backbone

• Vector backbone: pBABE Puro
• Backbone size w/o insert (bp): 5169
• Total vector size (bp): 9662
• Vector type: Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SHIP2
• Alt name: SHIP2; 51C protein
• Species: H. sapiens (human)
• Insert Size (bp): 4400
• GenBank ID: Y14385.1 NM_001567
• Entrez Gene: INPPL1 (a.k.a. OPSMD, SHIP2)
• Tag / Fusion Protein:
  • His Xpress (N-terminal)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: cagcttcaataaccctgcc
• 3′ sequencing primer: gaaagtgctggctggtga

Resource Information

• Supplemental Documents:
  • SHIP2 construct in pBABE

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry