RCAS-Flag-BRAFV600E
(Plasmid #126615)

• Purpose: Retroviral expression of Flag tagged human oncogenic BRAFV600E
• Depositing Lab: Yi Li
• Publication: Bu et al Cancer Res. 2019 Jan 1;79(1):61-71. doi: 10.1158/0008-5472.CAN-18-1087. Epub 2018 Nov 6.

Backbone

• Vector backbone: RCAS-Y
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BRAF
• Alt name: B-Raf proto-oncogene serine/threonine-protein kinase
• Species: H. sapiens (human)
• Mutation: V600E
• Entrez Gene: BRAF (a.k.a. B-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (unknown if destroyed)
• 3′ cloning site: ClaI (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: A PacI-ClaI PCR fragment of Flag-tagged BRAFV600E from Addgene plasmid # 53156 was inserted into RCAS-Y.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry