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pHAGE-CMV-hAAT-W
(Plasmid #126704)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 126704 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHAGE
  • Backbone manufacturer
    Derived in Kotton lab
  • Backbone size w/o insert (bp) 6431
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hAAT
  • Alt name
    SERPINA1
  • Alt name
    a1AT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1254
  • Entrez Gene
    SERPINA1 (a.k.a. A1A, A1AT, AAT, PI, PI1, PRO2275, alpha1AT, nNIF)
  • Promoter CMVp

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE-CMV-hAAT-W was a gift from Darrell Kotton (Addgene plasmid # 126704 ; http://n2t.net/addgene:126704 ; RRID:Addgene_126704)
  • For your References section:

    Amelioration of emphysema in mice through lentiviral transduction of long-lived pulmonary alveolar macrophages. Wilson AA, Murphy GJ, Hamakawa H, Kwok LW, Srinivasan S, Hovav AH, Mulligan RC, Amar S, Suki B, Kotton DN. J Clin Invest. 2010 Jan . 120(1):379-89. 10.1172/JCI36666 PubMed 20038801