pH7m34GW_RcCHAD-mCherry
(Plasmid #127784)

• Purpose: RcCHAD-mCherry expression in plants
• Depositing Lab: Michael Hothorn
• Publication: Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii: 2/3/e201900385. doi: 10.26508/lsa.201900385. Print 2019 Jun.

Backbone

• Vector backbone: pH7m34GW
• Backbone size w/o insert (bp): 8913
• Total vector size (bp): 12564
• Vector type: Plant Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Ubi10p:RcCHAD-mCherry
• Alt name: B9T8Q5
• Species: Ricinus communis
• Insert Size (bp): 3651
• GenBank ID: RCOM_0386350 EEF27758.1
• Promoter: Ubiquitin10 promoter + 5' UTR
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: GTAAAACGACGGCCAGT
• 3′ sequencing primer: AACAGCTATGACCATG

Resource Information

• Supplemental Documents:
  • pH7_Ubi10-RcCHAD-mCherry.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry