pSB1K3_pBen-sfGFP_J23101-mRFP
(Plasmid
#128126)
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PurposeExpressing sfGFP under control of benzoate responsive promoter (pBen) and expressing mRFP under constitutive promoter J23101, and RBS B0032
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128126 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSB1K3 (iGEM registry)
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesfGFP
- Promoter pBen (benzoate responsive promoter)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI, golden gate (unknown if destroyed)
- 3′ cloning site BsaI, golden gate (unknown if destroyed)
- 5′ sequencing primer GCTGAGTTGAAGGATCA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSB1K3_pBen-sfGFP_J23101-mRFP was a gift from Jean-Loup Faulon (Addgene plasmid # 128126 ; http://n2t.net/addgene:128126 ; RRID:Addgene_128126) -
For your References section:
Metabolic perceptrons for neural computing in biological systems. Pandi A, Koch M, Voyvodic PL, Soudier P, Bonnet J, Kushwaha M, Faulon JL. Nat Commun. 2019 Aug 28;10(1):3880. doi: 10.1038/s41467-019-11889-0. 10.1038/s41467-019-11889-0 PubMed 31462649
Map uploaded by the depositor.
