pFW11-ffluc
(Plasmid #128487)

• Purpose: Allelic exchange plasmid to to integrate firefly luciferase gene into S. pyogenes for biophotonic imaging
• Depositing Lab: Thomas Proft
• Depositing Organization: University of Auckland
  Located in New Zealand
• Publication: Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(22):9737-45. doi: 10.1007/s00253-013-5200-7. Epub 2013 Sep 6.

Backbone

• Vector backbone: pFW11
• Vector type: allelic replacement vector

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: firefly luciferase gene
• Alt name: ffluc
• Alt name: ffn
• Species: Photinus pyralis
• Insert Size (bp): 1653
• GenBank ID: AB644228.1 BAL46512.1
• Promoter: synthetic
• Tags / Fusion Proteins:
  • spy0535 upstream flanking region
  • spy0535 downstream flanking region

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (destroyed during cloning)
• 3′ cloning site: EcoRI (destroyed during cloning)
• 5′ sequencing primer: gaatggactaatgaaaatg
• 3′ sequencing primer: tagaatgaatatttccc

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Prof. Andreas Podbielski, University of Rostock, Germany. Please cite https://www.ncbi.nlm.nih.gov/pubmed/?term=podbielski+a+pFW in any work using this plasmid

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 128487-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 128487-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.