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pLZ12Km2-P23R:TA:ffluc
(Plasmid #88900)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 88900 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLZ12Km2
  • Backbone manufacturer
    Nobuhiko Okada (PMID: 9846743)
  • Vector type
    Bacterial Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    toxin-antitoxin + firefly luciferase
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer Unknown
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The P23 promoter sequence was cloned from pOri23 (Philippe Moreillon; PMID: 10816506). The toxin-antitoxin cassette was cloned from pBT286 (Urszula Zielenkiewicz; PMID: 16109951).
  • Terms and Licenses

Depositor Comments

This plasmid contains a toxin-antitoxin system that stabilizes the plasmid in Streptococcus by post-segregational killing of bacteria that have lost the plasmid. This stabilization improves plasmid utility in animal infection models. For more information, please see the original reference. Although designed for use with Streptococcus pyogenes, this plasmid also labels other streptococcal species. For more information, please see Loh JM and Proft T (2016). PMID: 26410784

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLZ12Km2-P23R:TA:ffluc was a gift from Thomas Proft (Addgene plasmid # 88900 ; http://n2t.net/addgene:88900 ; RRID:Addgene_88900)
  • For your References section:

    Toxin-antitoxin-stabilized reporter plasmids for biophotonic imaging of Group A streptococcus. Loh JM, Proft T. Appl Microbiol Biotechnol. 2013 Nov;97(22):9737-45. doi: 10.1007/s00253-013-5200-7. Epub 2013 Sep 6. 10.1007/s00253-013-5200-7 PubMed 24061415