pFW11-ffluc
(Plasmid #128487)

• Purpose: Allelic exchange plasmid to to integrate firefly luciferase gene into S. pyogenes for biophotonic imaging
• Depositing Lab: Thomas Proft
• Publication: Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(22):9737-45. doi: 10.1007/s00253-013-5200-7. Epub 2013 Sep 6.

Backbone

• Vector backbone: pFW11
• Vector type: allelic replacement vector

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: firefly luciferase gene
• Alt name: ffluc
• Alt name: ffn
• Species: Photinus pyralis
• Insert Size (bp): 1653
• GenBank ID: AB644228.1 BAL46512.1
• Promoter: synthetic
• Tags / Fusion Proteins:
  • spy0535 upstream flanking region
  • spy0535 downstream flanking region

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (destroyed during cloning)
• 3′ cloning site: EcoRI (destroyed during cloning)
• 5′ sequencing primer: gaatggactaatgaaaatg
• 3′ sequencing primer: tagaatgaatatttccc

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Prof. Andreas Podbielski, University of Rostock, Germany. Please cite https://www.ncbi.nlm.nih.gov/pubmed/?term=podbielski+a+pFW in any work using this plasmid

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry