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(Plasmid #88902)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 88902 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Nobuhiko Okada (PMID: 9846743)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    toxin-antitoxin + LuxABCDE
  • Species

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The P23 promoter sequence was cloned from pOri23 (Philippe Moreillon; PMID: 10816506). The toxin-antitoxin cassette was cloned from pBT286 (Urszula Zielenkiewicz; PMID: 16109951).
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

This plasmid contains a toxin-antitoxin system that stabilizes the plasmid in Streptococcus by post-segregational killing of bacteria that have lost the plasmid. This stabilization improves plasmid utility in animal infection models. For more information, please see the original reference. Although designed for use with Streptococcus pyogenes, this plasmid also labels other streptococcal species. For more information, please see Loh JM and Proft T (2016). PMID: 26410784

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLZ12Km2-P23R:TA:Xen5 was a gift from Thomas Proft (Addgene plasmid # 88902 ; ; RRID:Addgene_88902)
  • For your References section:

    Toxin-antitoxin-stabilized reporter plasmids for biophotonic imaging of Group A streptococcus. Loh JM, Proft T. Appl Microbiol Biotechnol. 2013 Nov;97(22):9737-45. doi: 10.1007/s00253-013-5200-7. Epub 2013 Sep 6. 10.1007/s00253-013-5200-7 PubMed 24061415